Results
| PMID | 25376627 |
| Gene Name | TBPL2 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 65 |
| Population details | 65 (31 patients with endometriosis, 34 patients without endometriosis) |
| Age | 30–39 yrs |
| Sex | Female |
| Infertility type | Female infertility |
| Associated genes | GPX3, IL6, IL8, TNF, TXN, TBPL2 |
| Other associated phenotypes |
Endometriosis |
|
Reproduction. 2015 Feb;149(2):155-62. doi: 10.1530/REP-14-0438. Epub 2014 Nov 5. Choi, Young Sik| Cho, SiHyun| Seo, Seok Kyo| Park, Joo Hyun| Kim, Seok Hyun| Lee, Byung Seok Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartmen The aim of this study was to compare intrafollicular biomarkers of thiol-redox system and chronic inflammation in infertile patients with and without endometriosis, and examine correlations between biomarkers and IVF outcomes. The study included 65 patients receiving IVF: 31 patients with endometriosis vs 34 patients without endometriosis. Follicular fluid (FF) was obtained from a single-dominant follicle during oocyte retrieval and stored at -70 degrees C. Malondialdehyde, superoxide dismutase, glutathione (GSH), glutathione peroxidase 3 (GPX3), thioredoxin (TRX), TRX-binding protein 2 (TBP2), and peroxiredoxin-4 levels were measured in the FF samples by ELISAs as biomarkers of oxidative stress. The inflammatory cytokines interleukin 1 beta (IL1beta), IL6, IL8, and tumor-necrosis factor alpha (TNFalpha) were also measured by ELISAs. GSH levels were significantly lower in the endometriosis group compared with the controls. TBP2 levels were significantly higher in the endometriosis group. IL6, IL8, and TNFalpha levels were significantly higher in the endometriosis group. The levels of all of the inflammatory cytokines positively correlated with the levels of TRX. GSH levels positively correlated with the number of high-quality embryos. GPX3 and TRX levels negatively correlated with the percentage of mature oocytes. TNFalpha levels negatively correlated with the cumulative embryo score per embryo. Logistic regression analysis revealed that the number of high-quality embryos was an independent factor predicting clinical pregnancy. In conclusion, there may be an imbalance in the thiol-redox system and increased levels of inflammatory cytokines in the intrafollicular microenvironment of infertile patients with endometriosis, which may affect the qualities of the oocyte and embryo. Mesh Terms: Adult| Biomarkers/analysis| Cytokines/analysis| Endometriosis/*metabolism/surgery| Female| Fertilization in Vitro| Follicular Fluid/chemistry| Humans| Infertility, Female/*metabolism| Inflammation/metabolism| Ovarian Follicle/*metabolism| Ovula |
|